UI10-00038 - SRA

SRX315450: UI10-00038
1 ILLUMINA (Illumina HiSeq 2000) run: 2.5M spots, 196.9M bases, 173.9Mb downloads

Design: "To detect variation and population structure in a broad sample of Miscanthus sinensis, genotyping-by-sequencing was performed with the following protocol: DNA was digested with PstI and MspI and ligated to barcoded PstI adapters and a universal MspI adapter. 95 samples were pooled to create one library. Size selection of 200-500 bp was performed on a 2% agarose gel. After sequencing, the software Stacks was used to place reads into separate files by barcode, remove barcode sequence, trim the remaining sequence to 80 nt, and filter out low quality reads and reads with uncalled bases."

Submitted by: University of Illinois, Urbana-Champaign

Study: Genetic diversity of Miscanthus sinensis

PRJNA207721 • SRP026347 • All experiments • All runs

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Genotyping-by-sequencing of Miscanthus sinensis accessions from the native range in Asia and from nurseries and naturalized populations in the United States. Several other species are included in order to infer species relationships and detect hybridization.

Sample: Generic sample from Miscanthus sinensis var. condensatus

SAMN02209258 • SRS452858 • All experiments • All runs

Organism: Miscanthus sinensis subsp. condensatus

Library:

Name: UI10-00038

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: Reduced Representation

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 2.5M spots, 196.9M bases, 173.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR922663	2,460,984	196.9M	173.9Mb	2014-06-26

ID:: 440584

SRA

Sequence Read Archive

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